STUDIUL POLIZAHARIDELOR PRODUSE DE CIANOBACTERIA SPIRULINA PLATENSIS LA CULTIVARE MIXOTROFĂ

Valentina BULIMAGA, Liliana ZOSIM, Alina TROFIM, Veaceslav REVA, Maria-Bianca BULIMAGA

Abstract


În prezentul studiu a fost evaluat efectul  acetaţilor de Cu(II) şi Zn(II) cu şi fără adaos de glucoză şi al iluminării asupra productivităţii şi producerii polizaharidelor intracelulare la cianobacteria Spirulina platensis. A fost analizată componenţa fracţiilor polizaharidice obţinute la separare prin cromatografia pe coloana de Toyo-Pearl şi stabilită natura lor proteoglicanică. A fost propusă schema  de realizare a unui procedeu de extragere şi purificare a complexului de polizaharide din biomasă.

 

STUDY OF POLYSACHARIDES PRODUCED BY CYANOBACTERIA
      SPIRULINA PLATENSIS IN MIXTROPHIC CULTIVATION

In the present work, the authors studied the effect of Cu (II) and Zn (II) acetates with and without addition of glucose and the influence of two illumination regimes on the productivity and production of intracellular polysaccharides in cyanobacterium Spirulina platensis. The composition of the polysaccharide fractions obtained by chromatographic separation on a Toyo-Pearl column was analyzed and their proteoglycanic nature was determined. A scheme for the extraction and purification procedure of the polysaccharide complex from biomass has been proposed.


Keywords


Spirulina platensis, cultivation, intracellular polysaccharides, extraction, purification

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