СНИЖЕНИЕ АЛЛЕРГЕННОСТИ БЕЛКОВ НЕОЧИЩЕННЫХ ЭКСТРАКТОВ СЕМЯН АРАХИСА И СОИ МЕТОДОМ ОГРАНИЧЕННОГО ПРОТЕОЛИЗА

Ala CHERDIVARĂ, Андрей ШУТОВ

Abstract


Proteoliza limitată cu papaină a extractelor proteinelor nepurificate din seminţele de arahide şi soia duce la scindarea regiunii C-terminale a domeniilor N-terminale ale globulinelor 11S, precum şi a regiunii N-terminale a globulinei 7S din arahide. În aceste domenii au fost identificaţi epitopii IgE, care determină în mare măsură alergenicitatea globuline­lor 11S şi 7S din arahide şi soia. Rezultatele obţinute indică posibilitatea fundamentală de reducere a alergenicităţii proteinelor din arahide şi soia atunci când se utilizează ca substraturi nu doar preparatele purificate de globuline, aşa cum s-a stabilit anterior, dar şi extractele de proteine totale din seminţe.

 

LIMITED PROTEOLYSIS OF PROTEINS FROM NON-PURIFIED EXTRACTS OF PEANUT AND

SOYBEAN SEEDS REDUCES THEIR ALLERGENICITY

Limited papain proteolysis of unpurified protein extracts from peanut and soybean seeds leads to cleavage off of the C-terminal region of the N-terminal domains of 11S globulins, as well as the N-terminal region of peanut 7S globulin. In these regions, IgE epitopes have been identified, largely determining the allergenicity of 11S and 7S peanut and soybean globulins. The results indicate the fundamental possibility of reducing the allergenicity of peanut and soybean proteins when using not only purified globulin preparations as substrates, as established earlier, but also total protein extracts of seeds.


Keywords


seed storage globulins, allergens, IgE epitopes, peanut, soybean.

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References


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