VIABILITY AND ANTIMICROBIAL ACTIVITY OF STREPTOMYCES STRAINS FROM NCNM AFTER LYOPHILIZATION

Oleg CHISELIŢA, Svetlana BURŢEVA, Maxim BÎRSA, Valentina BULIMAGA, Anastasia VASILICIUC

Abstract


The article deals with the aspects related to lyophilization of streptomycetes strains, preserved in the National Collection of Nonpathogenic Microorganisms (NCNM). Was determined that lyophilization do not significantly modify the antimicrobial activity of streptomycetes. Maximum viability of strains of genus Streptomyces (83,2-90,2%) is ensured after lyophilization at initial titer by 9-11 log10UFC ml-1 in protective medium (gelatin 2,5% + glucose 7,5%) by rehydra­tion with distillate water.

VIABILITATEA ŞI ACTIVITATEA ANTIMICROBIANĂ A TULPINELOR DE STREPTOMYCES
DIN CNMN DUPĂ LIOFILIZARE

Acest articol prezintă aspecte legate de liofilizarea tulpinilor de streptomicete, depozitate în Colecţia Naţională de Microorganisme Nepatogene (CNMN). A fost stabilit că liofilizarea nu modifică esenţial activitatea antimicrobiană a streptomicetelor. Viabilitatea maximă a tulpinilor genului Streptomyces (83,2-90,2%) este asigurată după liofilizarea la titrul iniţial 9-11 log10UFC ml-1 în mediu protectiv (gelatină 2,5% + glucosă 7,5%) şi la rehidratarea cu apă distilată.

 


Keywords


Streptomyces, lyophilization, cryoprotectants, viability, antimicrobial activity

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References


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