EVALUAREA SPECTRULUI DE PEPTIDE LA FRACŢIONAREA PRIN METODE CROMATOGRAFICE A HIDROLIZATELOR ENZIMATICE ALE FICOCIANINEI ŞI Se-FICOCIANINEI

Angela RUDAKOVA, Valentina BULIMAGA, Maria PISOVA, Veaceslav REVA, Natalia CLIMOVA, Sergiu RUDAKOV, Liliana ZOSIM

Abstract


A fost efectuată evaluarea comparativă a hidrolizatelor papainice şi triptice ale ficocianinei şi Se-ficocianinei în urma separării prin metoda HPLC cu faze inverse pe coloana de ResourceTMRPC. A fost analizat profilul cromatografic al hidrolizatelor papainice şi triptice ale ficocianinei la separare prin gel-filtrare pe coloana de sefadex G-50. Spectrele UV-VIS ale fracţiilor peptidice obţinute la separarea prin gel-filtrare a hidrolizatelor ficocianinei au scos în evidenţă pre­zenţa peptidelor legate cu cromoforul.

EVALUATION OF PEPTIDES SPECTRUM AT FRACTIONATION OF ENZYMATIC HYDROLYSATES OF PHYCOCYANIN AND Se-PHYCOCYANIN BY CHROMATOGRAPHIC METHODS

A comparative evaluation of papain and trypsin hydrolysates of phycocyanin and Se-phycocyanin after separation by HPLC with reverse phase on the ResourceTMRPC column was performed.The chromatographic profile of peptides from the trypsin and papain hydrolysates of phycocyanin at separation by gel filtration on Sephadex G-50 column was analyzed. UV-VIS spectra of peptides fractions obtained by gel filtration separation of phycocyanin hydrolysates revealed the presence of chromopeptides.


Keywords


phycocyanin, Se-phycocyanin, hydrolisis, peptides, separation.

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References


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