SINTEZA ORIENTATĂ A POLIZAHARIDELOR ACIDE LA CIANOBACTERIA SPIRULINA PLATENSIS CNM CB-02

Valentina BULIMAGA, Maria PISOVA, Liliana ZOSIM

Abstract


 Cultivarea cianobacteriei Spirulina platensis CNM CB-02 în două etape cu adaos de NaCl (0,25 şi 0,50 M) la a 2-a etapă de cultivare, fără a varia regimul de iluminare, sporeşte conţinutul de polizaharide sulfatate în biomasă cu 29 şi, respectiv, 31,3%, comparativ cu proba de referinţă (fără NaCl). În acest caz n-a fost observat un efect evident al acestei sări asupra conţinutului total de polizaharide acide. Majorarea intensităţii de iluminare (până la 5500 lx) şi supli­men­tarea cu NaCl (0,25 M) la a 2-a etapă de cultivare a asigurat un spor cu 23,45% al conţinutului total de polizaharide acide, com­parativ cu conţinutul acestora în proba de referinţă (cultivată la 3500 lx). Adăugarea CuSO4·5H2O (1-4 mg/l) nu a exer­citat un efect semnificativ asupra conţinutului de polizaharide acide şi sulfatate în biomasa de spirulină, însă a stimulat considerabil producerea de exopolizaharide acide (până la 238,3 g/kg) la 5500 lx.

ACIDIC POLYSACCHARIDES DIRECTED SYNTHESIS AT CYANOBACTERIA SPIRULINA PLATENSIS CNM CB-02

Two-stage cultivation of cyanobacterium Spirulina platensis CNM CB-02 with NaCl (0,25 and 0,50 M) supple­men­tation on the second stage of cultivation, without varying of the lighting regime ensured an increasing of the content of sulfated polysaccharides in biomass by 29 and 31,3%, respectively, compared to the reference sample (without NaCl). It wasn’t found an evident effect of NaCl on the total content of acidic polysaccharides in this case. Light intensity increasing (up to 5500 lx) with NaCl (0,25 M) supplementation ensured the increasing of the acidic polysaccharides content with 23,45%, compared to the reference sample (cultivated at 3500lx). CuSO4·5H2O (1-4 mg/l) hadn’t a significant effect on the content of acidic and sulfated polysaccharides in spirulina biomass, but considerably stimulated acidic exopoly­saccharides production (up to238,3 g / kg) at 5500 lx.


Keywords


spirulina, two-stage cultivation, stress factors, acidic intracellular polysaccharides and exopolysaccharides.

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